Journal: Immunity

Article Title: Signaling through the inhibitory Fc receptor FcγRIIB induces CD8 T cell apoptosis to limit T cell immunity

doi: 10.1016/j.immuni.2019.12.006

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Dr. Mark Cragg InVivoMAb anti-mouse CD16/32 (clone 2.4G2) BioXcell Cat# BE0307, RRID:AB_2736987 Bacterial and Virus Strains Biological Samples Emory Transplant Center Biorepository Emory IRB protocol #00046593 Chemicals, Peptides, and Recombinant Proteins GolgiPlug BD Biosciences Cat# 555029 OVA peptide 257–264 (SIINFEKL) GenScript Cat# RP10611 CTLA-4Ig (abatacept) Bristol-Myers Squibb, Abatacept (Orencia) Anti-CD28 domain antibodies Bristol-Myers Squibb Collagenase (type 1A) Sigma-Aldrich C2654 Hyaluronidase (type 1) Sigma-Aldrich H3506 Collagenase P Sigma-Aldrich Ref# 11213865001 Collagenase D Sigma-Aldrich Ref# 1088866001 Recombinant Fgl2 R&D Systems R&D Cat# 5257-FL-050 Critical Commercial Assays MACS CD8a+ T cell isolation kit, mouse Miltenyi Biotec 130-104-075 Quick-RNA MicroPrep Kit Zyma Research R1051 SMART-seq v4 cDNA synthesis kit Takara Cat# 634894 NexteraXT kit Illumina FC-131–1096 Mouse Fgl2 ELISA Biolegend Biolegend Cat# 437807 RNeasy Plus Micro Kit Qiagen Qiagen Cat# 74034 High Capacity cDNA reverse transcription kit ThermoFisher ThermoFisher Cat# 4368814 HT HG-U133 Plus PM BeadChip Affymetrix Thermo Fisher Cat# 901261 Lightning Link R-PE Antibody Labeling kit Novus Biologicals Cat # 703–0010 CountBright Beads Life Technologies Cat # C36950 Deposited Data RNAseq data NCBI Gene Expression Omnibus (GEO) GSE118439 Experimental Models: Cell Lines B16 Melanoma-OVA Brown, et al., 2001 , provided by Dr. Yang-Xin Fu Experimental Models: Organisms/Strains NCI C57Bl/6Ncr (NCI grantee program) Charles River Stock #556 NCI B6-LY5.1/Cr (NCI grantee program) Charles River Stock #564 OT-I Hogquist et al., 1994 OT-II Barnden et al., 1998 mOVA (C57Bl/6 background, H-2b) Ehst et al., 2013 Dr. Marc Jenkins B6.129P2-Aicda tm1(cre)Mnz /J ( Aicda −/− ) The Jackson Laboratory Stock #007770 B6;129S-Fcgr2btm1Ttk/J ( Fcgr2b −/− ) The Jackson Laboratory Stock #002848 EM:06078 Fcgr2b Fcgr2bB6null B6(Cg)-Fcgr2btm12Sjv/Cnbc ( Fcgr2b −/− ) European Mutant Mouse Archive; Dr. J.S.

Techniques: Control, Virus, Recombinant, Cell Isolation, cDNA Synthesis, Enzyme-linked Immunosorbent Assay, Reverse Transcription, Antibody Labeling, Gene Expression, Mutagenesis, Software

Journal: Biochimica et biophysica acta. Molecular basis of disease

Article Title: Integrated multiomic analysis identifies TRIP13 as a mediator of alveolar epithelial type II cell dysfunction in idiopathic pulmonary fibrosis

doi: 10.1016/j.bbadis.2024.167572

Figure Lengend Snippet: Key resources.

Article Snippet: Mouse IgG1 anti-human CD326 (EpCAM)-conjugated microbeads , Miltenyi Biotec , Cat# 130–061–101 RRID: AB_2832928.

Techniques: Plasmid Preparation, Magnetic Beads, Recombinant, Blocking Assay, Lysis, Protease Inhibitor, Red Blood Cell Lysis, Software, Microscopy, Fluorescence, Imaging

Journal: Cell Death & Disease

Article Title: Aldolase B-driven lactagenesis and CEACAM6 activation promote cell renewal and chemoresistance in colorectal cancer through the Warburg effect

doi: 10.1038/s41419-023-06187-z

Figure Lengend Snippet: A Metabolomic analysis based on nuclear magnetic resonance (NMR) showing metabolite levels in CRC cells with or without ALDOB overexpression. Black dashed lines indicate relative metabolite levels in control-treated cells. B Lactate levels in medium from cells with control or ALDOB overexpression. C Transwell-based co-culture system for assessment of cell growth (left panel) and chemoresistance (right panels). D Supplementation of medium from cells with or without ALDOB overexpression for assessment of cell proliferation (left panel) and chemoresistance (right panels). E Western blots demonstrating levels of the indicated proteins in cells with or without ALDOB overexpression. The statistical analysis is displayed in the right panel. F Immunofluorescence assay showing the expression of exogenous ALDOB (ALDOB-MYC) and LDHB in CRC cells transfected with ALDOB expression plasmid. The scale bar represents 20 μm. G Western blots showing the levels of the indicated proteins in cells treated with different concentrations of lactate. H Immunofluorescence assay showing LDHB expression in CRC cells treated with indicated concentrations of lactate. The scale bar represents 20 μm. All P values were obtained using the paired two-tailed Student’s t -test. * P < 0.05; ** P < 0.01; *** P < 0.001.

Article Snippet: A mouse monoclonal antibody targeting the MYC tag (Proteintech, Cat. 60003-2-Ig), and a rabbit monoclonal antibody to LDHB (Proteintech, Cat. 14824-1-AP) were used at a 1:200 dilution.

Techniques: Nuclear Magnetic Resonance, Over Expression, Control, Co-Culture Assay, Western Blot, Immunofluorescence, Expressing, Transfection, Plasmid Preparation, Two Tailed Test

Journal: eLife

Article Title: The conserved centrosomin motif, γTuNA, forms a dimer that directly activates microtubule nucleation by the γ-tubulin ring complex (γTuRC)

doi: 10.7554/eLife.80053

Figure Lengend Snippet:

Article Snippet: Antibody , anti-MZT1, (Rabbit polyclonal) , Abcam , ab178359 , 375 µg/mL, used in bead attachment assay.

Techniques: Clone Assay, Expressing, Recombinant, Plasmid Preparation, Modification, Magnetic Beads, Western Blot, Software

Journal: Cell reports

Article Title: Circadian Clock Regulation of Hepatic Lipid Metabolism by Modulation of m 6 A mRNA Methylation

doi: 10.1016/j.celrep.2018.10.068

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Mouse monoclonal anti- beta Actin , Abcam , ab8226; RRID: AB_306371.

Techniques: Recombinant, Cholesterol Assay, Isolation, Magnetic Beads, Software